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KMID : 1134820100390010031
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Journal of the Korean Society of Food Science and Nutrition
2010 Volume.39 No. 1 p.31 ~ p.35
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Helianthus tuberosus Extract Has Anti-Diabetes Effects in HIT-T15 Cells
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Kim Jeong-Lan
Bae Cho-Rong
Cha Youn-Soo
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Abstract
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This study was designed to evaluate anti-diabetes effect of Helianthus tuberosus extract (HT) in HIT-T15 cells. There were 5 experimental groups according to treatment NC (0 μL/mL), HT2 (1.1 μL/mL), HT3 (1.5 μL/mL), IN2 (1.8 μL/mL), IN3 (2.5 μL/mL). Inulin (IN) was used as a positive control for the Helianthus tuberosus extract groups. Cell viability was significantly increased in the HT3 (1.5 μL/mL), IN2 (1.8 μL/mL), IN3 (2.5 μL/mL) groups, compared with the NC group. There was no significant difference in cytotoxicity among all groups. Cell survival by MTT assay with alloxan was significantly increased in the HT2 (1.1 μL/mL), HT3 (1.5 μL/mL) groups, compared with the NC group. Insulin secretion and NAD?/NADH ratio were significantly increased in the HT3 group, compared with the NC group. We found that Helianthus tuberosus extract increased cell viability, had a protective effect on β-cells, and increased insulin secretion level and NAD+/NADH ratio in HIT-T15 cells. These results suggest that Helianthus tuberosus extract improves the diabetes-related factors.
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KEYWORD
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Helianthus tuberosus, anti-diabetes, HIT-T15 cells
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